Open AccessObservation of DNA damage of human hepatoma cells irradiated by heavy ions using comet assay
Author(s) -
Limei Qiu,
Wenjian Li,
Xinyue Pang,
Gao Qingxiang,
Yan Feng,
Libin Zhou,
Zhang Gaohua
Publication year - 2003
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v9.i7.1450
Subject(s) - comet assay , dna damage , irradiation , gel electrophoresis , comet , microbiology and biotechnology , ion , chemistry , physics , radiochemistry , dna , biology , biochemistry , nuclear physics , astrophysics , organic chemistry
Now many countries have developed cancer therapy with heavy ions, especially in GSI (Gesellschaft f r Schwerionenforschung mbH, Darmstadt, Germany), remarkable results have obtained, but due to the complexity of particle track structure, the basic theory still needs further researching. In this paper, the genotoxic effects of heavy ions irradiation on SMMC-7721 cells were measured using the single cell gel electrophoresis (comet assay). The information about the DNA damage made by other radiations such as X-ray, gamma-ray, UV and fast neutron irradiation is very plentiful, while little work have been done on the heavy ions so far. Hereby we tried to detect the reaction of liver cancer cells to heavy ion using comet assay, meanwhile to establish a database for clinic therapy of cancer with the heavy ions.