Open AccessGene-expression analysis of single cells-nested polymerase chain reaction after laser microdissection
Author(s) -
Xin Shi,
Jörg Kleeff,
Zhaowen Zhu,
Bruno M. Schmied,
Wenhao Tang,
Arthur Zimmermann,
Markus W. Büchler,
Helmut Friess
Publication year - 2003
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v9.i6.1337
Subject(s) - laser capture microdissection , microdissection , biology , complementary dna , microbiology and biotechnology , gene expression , nested polymerase chain reaction , polymerase chain reaction , gene , agarose gel electrophoresis , dna , rna , real time polymerase chain reaction , genetics
The structural and functional characteristics of cells are dependent on the specific gene expression profile. The ability to study and compare gene expression at the cellular level will therefore provide valuable insights into cell physiology and pathophysiology.