Open AccessEstablishment and characterization of a rat pancreatic stellate cell line by spontaneous immortalization
Author(s) -
Atsushi Masamune,
Masahiro Satoh,
Kazuhiro Kikuta,
Noriaki Suzuki,
Tooru Shimosegawa
Publication year - 2003
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v9.i12.2751
Subject(s) - hepatic stellate cell , biology , microbiology and biotechnology , mapk/erk pathway , cytokine , cell culture , kinase , immunology , endocrinology , genetics
Activated pancreatic stellate cells (PSCs) have been implicated in the pathogenesis of pancreatic fibrosis and inflammation. Primary PSCs can be subcultured only several times because of their limited growth potential. A continuous cell line may therefore be valuable in studying molecular mechanisms of these pancreatic disorders. The aim of this study was to establish a cell line of rat PSCs by spontaneous immortalization.