Open AccessHeterologous expression of human cytochrome P450 2E1 in HepG2 cell line
Author(s) -
Jian Zhuge,
Yuanfang Luo,
Yixun Yu
Publication year - 2003
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v9.i12.2732
Subject(s) - complementary dna , microbiology and biotechnology , cyp2e1 , biology , expression vector , recombinant dna , transfection , biochemistry , chemistry , cytochrome p450 , gene , enzyme
Human cytochrome P-450 2E1 (CYP2E1) takes part in the biotransformation of ethanol, acetone, many small-molecule substrates and volatile anesthetics. CYP2E1 is involved in chemical activation of many carcinogens, procarcinogens, and toxicants. To assess the metabolic and toxicological characteristics of CYP2E1, we cloned CYP2E1 cDNA and established a HepG2 cell line stably expressing recombinant CYP 2E1.