Open AccessAnalysis of Heme oxygenase isomers in rat
Author(s) -
Zhenwei Xia,
Wenjun Cui,
Xuehong Zhang,
Qing Shen,
Jian Wang,
Yun-Zhu Li,
Shen-Nian Chen,
Shiying Yu
Publication year - 2002
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v8.i6.1123
Subject(s) - microbiology and biotechnology , heme oxygenase , complementary dna , gel electrophoresis , biology , spleen , microsome , heme , polyacrylamide gel electrophoresis , biochemistry , enzyme , gene , immunology
To purify and identify heme oxygenase (HO) isomers which exist in rat liver, spleen and brain treated with hematin and phenylhydrazine and in untreated rat liver and to investigate the characteristics of HO isomers, to isolate and confirm the rat HO-1 cDNA that actually encodes HO-1 by expressing cDNA in monkey kidney cells (COS-1 cells), to prepare the rat heme oxygenase-1 (HO-1) mutant and to detect inhibition of HO-1 mutated enzyme.