Analysis of Heme oxygenase isomers in rat | Zendy

Zhenwei Xia | Zendy

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Analysis of Heme oxygenase isomers in rat

Author(s) -

Zhenwei Xia

Publication year - 2002

Publication title -

world journal of gastroenterology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.427

H-Index - 155

eISSN - 2219-2840

pISSN - 1007-9327

DOI - 10.3748/wjg.v8.i6.1123

Subject(s) - microbiology and biotechnology , heme oxygenase , complementary dna , gel electrophoresis , biology , spleen , microsome , heme , polyacrylamide gel electrophoresis , biochemistry , enzyme , gene , immunology

To purify and identify heme oxygenase (HO) isomers which exist in rat liver, spleen and brain treated with hematin and phenylhydrazine and in untreated rat liver and to investigate the characteristics of HO isomers, to isolate and confirm the rat HO-1 cDNA that actually encodes HO-1 by expressing cDNA in monkey kidney cells (COS-1 cells), to prepare the rat heme oxygenase-1 (HO-1) mutant and to detect inhibition of HO-1 mutated enzyme.

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