Open AccessEstablishment of an artificial β-cell line expressing insulin under the control of doxycycline
Author(s) -
Xinyu Qin,
Kuntang Shen,
Xin Zhang,
Zhi-hong Cheng,
Xiangru Xu,
ZeGuang Han
Publication year - 2002
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v8.i2.367
Subject(s) - proinsulin , microbiology and biotechnology , transfection , cell culture , biology , hygromycin b , doxycycline , plasmid , gene expression , insulin , expression vector , transactivation , complementary dna , beta cell , gene , recombinant dna , biochemistry , endocrinology , islet , antibiotics , genetics
Artificial beta-cell lines may offer an abundant source of cells for the treatment of type I diabetes, but insulin secretion in beta-cells is tightly regulated in physiological conditions. The Tet-On system is a "gene switch" system, which can induce gene expression by administration of tetracycline (Tet) derivatives such as doxcycline (Dox). Using this system, we established 293 cells to an artificial cell line secreting insulin in response to stimulation by Dox.