Open AccessCloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells
Author(s) -
Guonian Zhu,
Yixun Yu,
Xin Li,
Yuli Qian
Publication year - 2002
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v8.i2.318
Subject(s) - tolbutamide , complementary dna , microbiology and biotechnology , biology , biochemistry , expression vector , enzyme , molecular cloning , cyp2c9 , cdna library , recombinant dna , cytochrome p450 , affinity chromatography , gene , diabetes mellitus , endocrinology
Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2C9 cDNA was cloned and expressed stably in CHL cells.