Cloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells | Zendy

Zhu Ge-jian | Zendy; Yingnian Yu | Zendy; Xin Li | Zendy; Yuli Qian | Zendy

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Cloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells

Author(s) -

Zhu Ge-jian,

Yingnian Yu,

Xin Li,

Yuli Qian

Publication year - 2002

Publication title -

world journal of gastroenterology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.427

H-Index - 155

eISSN - 2219-2840

pISSN - 1007-9327

DOI - 10.3748/wjg.v8.i2.318

Subject(s) - tolbutamide , complementary dna , microbiology and biotechnology , biology , biochemistry , expression vector , enzyme , molecular cloning , cyp2c9 , cdna library , recombinant dna , cytochrome p450 , affinity chromatography , gene , diabetes mellitus , endocrinology

Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2C9 cDNA was cloned and expressed stably in CHL cells.

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