Open AccessSpecific CEA-producing colorectal carcinoma cell killing with recombinant adenoviral vector containing cytosine deaminase gene
Author(s) -
Lizong Shen,
Wei Wu,
Dehua Xu,
Zhiming Zheng,
Xinyuan Liu,
Qiang Ding,
Yibing Hua,
Kun Yao
Publication year - 2002
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v8.i2.270
Subject(s) - microbiology and biotechnology , cytosine deaminase , recombinant dna , cytotoxic t cell , viral vector , biology , virology , suicide gene , genetic enhancement , cell culture , hela , chemistry , gene , in vitro , biochemistry , genetics
To kill CEA positive colorectal carcinoma cells specifically using the E coli cytosine deaminase (CD) suicide gene, a new replication-deficient recombinant adenoviral vector was constructed in which CD gene was controlled under CEA promoter and its in vitro cytotoxic effects were evaluated.