Open AccessThree-dimensional image of hepatocellular carcinoma under confocal laser scanning microscope
Author(s) -
Wang-Hai Zhang,
Shuifang Zhu,
Shi-Lun Lu,
Ya-Lin Huang,
Peng Zhao
Publication year - 2000
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v6.i3.344
Subject(s) - confocal , confocal laser scanning microscopy , hepatocellular carcinoma , pathology , pleomorphism (cytology) , confocal microscopy , carcinoma , microscopy , biology , medicine , optics , biomedical engineering , immunohistochemistry , physics , cancer research , microbiology and biotechnology
AIM:To investigate the application of confocal laser scanning microscopy (CLSM) in tumor pathology and three-dimensional (3-D) reconstruction by CLSM in pathologic specimens of hepatocellular carcinoma (HCC).METHODS:The 30&mgr;m thick sections were cut from the paraffin embedded tissues of HCC, hyperplasia and normal liver,stained with DNA fluorescent probe YOYO-1 iodide and examined by CLSM to collect optical sections of nuclei and 3-D images reconstructed.RESULTS:HCC displayed chaotic arrangement of carcinoma cell nuclei, marked pleomorphism, indented and irregular nuclear surface, and irregular and coarse chromatin texture.CONCLUSION:The serial optical tomograms of CLSM can be used to create 3-D reconstruction of cancer cell nuclei. Such 3-D impressions might be helpful or even essential in making an accurate diagnosis.