Pathogenic effects of Opolysaccharide from Shigella flexneri strain | Zendy

Qi-Ping Zhong | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Pathogenic effects of Opolysaccharide from Shigella flexneri strain

Author(s) -

Qi-Ping Zhong

Publication year - 1999

Publication title -

world journal of gastroenterology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.427

H-Index - 155

eISSN - 2219-2840

pISSN - 1007-9327

DOI - 10.3748/wjg.v5.i3.245

Subject(s) - shigella flexneri , shigella , microbiology and biotechnology , in vivo , hela , in vitro , pathogenesis , chemistry , enterobacteriaceae , biology , escherichia coli , biochemistry , immunology , gene

AIM:To investigate the specific pathogenesis of O-polysaccharide (O-PS) which is on the outer membrane of lipopolysaccharides (LPS) from Shigella flexneri.METHODS:The O-PS was isolated and purified from Shigella flexneri 5 M90T by enzymatic hydrolysis and gel chromatography. Effects of O-PS were observed by in vitro experiment, (HeLa cell culture), and in vivo experiment (rabbit ileal loop assay).RESULTS:In vitro and in vivo experiments with the purified O-PS from Shigella flexneri revealed that the O-PS alone was toxic to Hela cells and caused mucosal inflammation and hemorrhagic exudation in ileal loop of rabbit.DISCUSSION:O-PS might be one of the factors causing diarrhea and its mechanism was different from endotoxin reaction of LPS.The molecular mechanism of O-PS need further studies.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research