Open AccessPathogenic effects of Opolysaccharide from Shigella flexneri strain
Author(s) -
Qi-Ping Zhong
Publication year - 1999
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v5.i3.245
Subject(s) - shigella flexneri , shigella , microbiology and biotechnology , in vivo , hela , in vitro , pathogenesis , chemistry , enterobacteriaceae , biology , escherichia coli , biochemistry , immunology , gene
AIM:To investigate the specific pathogenesis of O-polysaccharide (O-PS) which is on the outer membrane of lipopolysaccharides (LPS) from Shigella flexneri.METHODS:The O-PS was isolated and purified from Shigella flexneri 5 M90T by enzymatic hydrolysis and gel chromatography. Effects of O-PS were observed by in vitro experiment, (HeLa cell culture), and in vivo experiment (rabbit ileal loop assay).RESULTS:In vitro and in vivo experiments with the purified O-PS from Shigella flexneri revealed that the O-PS alone was toxic to Hela cells and caused mucosal inflammation and hemorrhagic exudation in ileal loop of rabbit.DISCUSSION:O-PS might be one of the factors causing diarrhea and its mechanism was different from endotoxin reaction of LPS.The molecular mechanism of O-PS need further studies.