Open AccessCloning and identification of an angiostatic molecule IP-10/crg-2
Author(s) -
Zhiguo Liu,
Jinghua Yang,
Hua-Zhang An,
Haiyan Wang,
Fengtian He,
Zheyi Han,
Ying Han,
WU Han-ping,
Bing Xiao,
Daiming Fan
Publication year - 1999
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v5.i3.241
Subject(s) - cloning (programming) , puc19 , microbiology and biotechnology , gene , biology , recombinant dna , molecular cloning , restriction enzyme , plasmid , complementary dna , genetics , computer science , programming language
AIM:To obtain human and murine cDNAs encoding IFN-gamma inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2).METHODS:The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-gamma and TNF-alpha,respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+).RESULTS:The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing.CONCLUSION:Recombinant IP-10/crg-2 gene clones with 306bp and 314bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2.