Cloning and identification of an angiostatic molecule IP-10/crg-2 | Zendy

Zhiguo Liu | Zendy

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Cloning and identification of an angiostatic molecule IP-10/crg-2

Author(s) -

Zhiguo Liu

Publication year - 1999

Publication title -

world journal of gastroenterology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.427

H-Index - 155

eISSN - 2219-2840

pISSN - 1007-9327

DOI - 10.3748/wjg.v5.i3.241

Subject(s) - cloning (programming) , puc19 , microbiology and biotechnology , gene , biology , recombinant dna , molecular cloning , restriction enzyme , plasmid , complementary dna , genetics , computer science , programming language

AIM:To obtain human and murine cDNAs encoding IFN-gamma inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2).METHODS:The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-gamma and TNF-alpha,respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+).RESULTS:The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing.CONCLUSION:Recombinant IP-10/crg-2 gene clones with 306bp and 314bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2.

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