Open AccessIn situhybridization assay of androgen receptor gene in hepatocarcinogenesis
Author(s) -
Guimin Zhao,
Ling Xue,
Hongyu Xu,
Ximing Tang,
Rui-De Hu,
Jun Dong
Publication year - 1998
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v4.i6.503
Subject(s) - in situ hybridization , androgen receptor , androgen , biology , gene expression , receptor , cancer research , medicine , endocrinology , pathology , gene , cancer , hormone , prostate cancer , biochemistry , genetics
AIM:To determine the correlation between expression of androgen receptor (AR) gene and hepatocarcinogenesis.METHODS:Male SD rats were used as experimental animals and the animal model of experimental hepatocarcinoma was established by means of 3'-me-DAB administration. Androgen receptor mRNA was detected by a non-radioactive in situ hybridization assay in neoplastic and non-neoplastic liver tissues.RESULTS:The expression of androgen receptor mRNA was observed only in neoplastic cells and some atypical hyperplastic cells. In the liver tissue of control animal and the remaining normal liver cells adjacent to the carcinoma tissue, no positive signal was seen.CONCLUSION:Androgen has an important correlation with hepatocarcinogenesis and the expression of androgen receptor gene might be a mark event during hepatocarcinogenesis.