Open AccessCodon 249 mutations ofp53gene in development of hepatocellular carcinoma
Author(s) -
Xinsheng Peng
Publication year - 1998
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v4.i2.125
Subject(s) - biology , gene , loss of heterozygosity , transcription (linguistics) , microbiology and biotechnology , hepatocellular carcinoma , mutation , gene mutation , genetics , messenger rna , gene expression , cancer research , allele , linguistics , philosophy
AIM:To investigate the mechanisms of codon 249 mutation of p53 gene in the formation of hepatocellular carcinoma (HCC).METHODS:Codon 249 mutation accompanied by loss of heterozygosity (LOH) and its effect on translation and transcription were studied using SSCP, IHC and RT-PCR/slot hybridization.RESULTS:Codon 249 mutations were detected in 32.9%, LOH detected in 68.4% among the HCC patients.Mutations of condon 249 were accompanied by LOH in 90%.The positive rates of p53 protein and mRNA were 91.3% and 95.7%, in mutational group, both were significantly higher than those in the non-mutational group (91.3% vs 19.1% and 95.7% vs 40.4%, respectively, both P < 0.01). The translation of p53 gene was strongly related to its transcription by correlation analysis( r = 0.8208).CONCLUSION:LOH might play an important role in hepatocarcinogenesis of codon 249 mutation, which could increase both transcription and translation of p53 gene.The increased expression of p53 protein mainly depend on the increased transcription of p53 gene.