Open AccessEstablishment of a nested-ASP-PCR method to determine the clarithromycin resistance ofHelicobacter pylori
Author(s) -
Xiaofeng Luo,
Jianhua Jiao,
Zhang Wen-yue,
Han-Ming Pu,
Bao-Jin Qu,
Bo Yang,
Min Hou,
Minjun Ji
Publication year - 2016
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v22.i25.5822
Subject(s) - nested polymerase chain reaction , saliva , genotype , helicobacter pylori , polymerase chain reaction , primer (cosmetics) , biology , microbiology and biotechnology , clarithromycin , gene , chemistry , genetics , biochemistry , organic chemistry
To investigate clarithromycin resistance positions 2142, 2143 and 2144 of the 23SrRNA gene in Helicobacter pylori (H. pylori) by nested-allele specific primer-polymerase chain reaction (nested-ASP-PCR).