Open AccessDevelopment ofFok-I based nested polymerase chain reaction-restriction fragment length polymorphism analysis for detection of hepatitis B virus X region V5M mutation
Author(s) -
Hong Kim
Publication year - 2015
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v21.i47.13360
Subject(s) - nested polymerase chain reaction , genotype , ethidium bromide , polymerase chain reaction , microbiology and biotechnology , amplicon , restriction fragment length polymorphism , agarose gel electrophoresis , restriction enzyme , biology , hepatocellular carcinoma , virology , hepatitis b virus , restriction digest , virus , dna , genetics , gene
To develop a Fok-I nested polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) method for the detection of hepatitis B virus X region (HBx) V5M mutation.