Open AccessPorcine hepatocyte isolation and reversible immortalization mediated by retroviral transfer and site-specific recombination
Author(s) -
Fan Meng,
Zhi Shui Chen,
Meng Han,
Xin Hu,
Xia He,
Yong Liu,
He We,
Wei Huang,
Hui Guo,
Ping Zhou
Publication year - 2010
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v16.i13.1660
Subject(s) - biology , microbiology and biotechnology , hepatocyte , viral vector , cell culture , clone (java method) , transfection , cre recombinase , immortalised cell line , gene , virology , transgene , genetics , recombinant dna , in vitro , genetically modified mouse
To develop a hepatocyte cell line, we immortalized primary porcine hepatocytes with a retroviral vector SSR#69 containing the Simian Virus 40 T antigen (SV40Tag).