Open AccessGFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line
Author(s) -
Gunter Maubach
Publication year - 2006
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v12.i5.723
Subject(s) - transgene , hepatic stellate cell , biology , microbiology and biotechnology , cell culture , transfection , genetically modified mouse , glial fibrillary acidic protein , gene expression , endogeny , downregulation and upregulation , gene , immunohistochemistry , immunology , genetics , biochemistry , endocrinology
The GFAP was traditionally considered to be a biomarker for neural glia (mainly astrocytes and non-myelinating Schwann cells). Genetically, a 2.2-kb human GFAP promoter has been successfully used to target astrocytes in vitro and in vivo. More recently, GFAP was also established as one of the several makers for identifying hepatic stellate cells (HSC). In this project, possible application of the same 2.2-kb human GFAP promoter for targeting HSC was investigated.