Open AccessCost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells
Author(s) -
Qian Zhou,
Baoqin Xuan,
Taishan Min,
Jianfeng Xu,
Lin Liu,
Weida Huang
Publication year - 2005
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v11.i9.1297
Subject(s) - microbiology and biotechnology , lipofectamine , transfection , hbsag , hepatitis b virus , small interfering rna , hbeag , biology , expression vector , virus , complementary dna , cell culture , virology , recombinant dna , gene , vector (molecular biology) , biochemistry , genetics
To find a cost-effective method of preparation of short interfering RNAs based on cloning, fermentation, digestion and purification (CFDP) and test its feasibility to inhibit hepatitis B virus replication in cell culture.