Open AccessLoss of heterozygosity analyzed by single nucleotide polymorphism array in cancer
Author(s) -
Haitao Zheng,
Zhihai Peng,
Sheng Li,
Lin He
Publication year - 2005
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v11.i43.6740
Subject(s) - loss of heterozygosity , single nucleotide polymorphism , biology , genetics , snp , microsatellite , genotype , snp array , tumor suppressor gene , gene , allele , cancer research , microbiology and biotechnology , carcinogenesis
Neoplastic progression is generally characterized by the accumulation of multiple genetic alterations including loss of tumor suppression gene function. Loss of heterozygosity (LOH) has been used to identify genomic regions that harbor tumor suppressor genes and to characterize different tumor types, pathological stages and progression. LOH pattern has been detected by allelotyping using restriction fragment length polymorphism, and later by simple sequence length polymorphisms (SSLPs or microsatellite) for 10 years. This paper reviews the detection of LOH by recently developed single nucleotide polymorphism (SNP) arrays (all analyzed by Affymetrix array); furthermore, its advantage and disadvantage were analyzed in several kinds of cancer.