Open AccessConstruction of prokaryotic expression system of TGF-β1epitope gene and identification of recombinant fusion protein immunity
Author(s) -
Yi Guo,
Zhiming Hao,
Jinyan Luo,
Junhong Wang
Publication year - 2005
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v11.i40.6389
Subject(s) - microbiology and biotechnology , hbcag , antigenicity , epitope , recombinant dna , biology , fusion protein , restriction enzyme , expression vector , gene , virology , chemistry , antigen , hepatitis b virus , hbsag , biochemistry , virus , genetics
To insert the constructed TGF-beta(1) epitope gene into the el loop of C-terminus of truncated hepatitis B core antigen to increase TGF-beta(1) antigenicity in its prokaryotic expression system and to identify immunity of the expressed recombinant protein in order to exploit the possibility for obtaining anti-TGF-beta(1) vaccine.