Open AccessConstruction, expression and characterization of human interferon α2b-(G4S)n-thymosin α1 fusion proteins inPichia pastoris
Author(s) -
Youfeng Yang,
Hao Yuan,
Nansong Liu,
Xiangling Chen,
Gao Buyu,
Hong Lü,
Yuyang Li
Publication year - 2005
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v11.i17.2597
Subject(s) - pichia pastoris , fusion protein , biology , microbiology and biotechnology , affinity chromatography , recombinant dna , virology , gene , biochemistry , enzyme
Interferon alpha2b (IFNalpha2b) and thymosin alpha1 (Talpha1) exhibit synergic effects in the treatment of hepatitis B and hepatitis C when used together. For developing a fusion protein drug, fusion proteins of IFNalpha2b and Talpha1 linked by different lengths of (G4S)n (n = 1-3) were constructed and expressed in Pichia pastoris.