Open AccessInsulin expression in livers of diabetic mice mediated by hydrodynamics-based administration
Author(s) -
Chenxia He,
Ding Shi,
Wenjun Wu,
Youfa Ding,
Deng-Min Feng,
Bin Lü,
Hao Ming Chen,
Junxia Yao,
Qi Shen,
Da-Yong Lu,
Jinglun Xue
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i4.567
Subject(s) - insulin , medicine , endocrinology , diabetes mellitus , glycemic , streptozotocin , type 1 diabetes , basal (medicine) , downregulation and upregulation , immunostaining , biology , immunohistochemistry , gene , biochemistry
Transfer and expression of insulin gene in vivo are an alternative strategy to improve glycemic control in type 1 diabetes. Hydrodynamics-based procedure has been proved to be very efficient to transfer naked DNA to mouse livers. The basal hepatic insulin production mediated by this rapid tail vein injection was studied to determine its effect on the resumption of glycemic control in type 1 diabetic mice.