Open AccessHigh-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeastPichia pastoris
Author(s) -
RuShi Liu
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i24.3602
Subject(s) - pichia pastoris , recombinant dna , microbiology and biotechnology , biology , plasmid , pichia , antigenicity , expression vector , complementary dna , coronavirus , electroporation , transformation (genetics) , virology , gene , antigen , biochemistry , genetics , covid-19 , medicine , disease , pathology , infectious disease (medical specialty)
Nucleocapsid (N) protein plays an important role in reproduction and pathological reaction of severe acute respiratory syndrome (SARS) coronavirus (SCoV), the antigenicity of the protein is better than spike (S) protein. This study was to find a highly specific and antigenic recombinant SCoV nucleocapsid (rSCoVN) protein, and to provide a basis for further researches on early diagnosis of SARS.