Open AccessMicroarray-based method for detecting methylation changes of p16Ink4agene 5’-CpG islands in gastric carcinomas
Author(s) -
Peng Hou,
Jia-Yao Shen,
Meiju Ji,
gyue He,
Zhaohui Lü
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i24.3553
Subject(s) - dna methylation , cpg site , methylation , biology , illumina methylation assay , methylated dna immunoprecipitation , bisulfite sequencing , microbiology and biotechnology , epigenetics , differentially methylated regions , dna microarray , carcinogenesis , microarray , gene , cancer research , genetics , gene expression
Aberrant DNA methylation of CpG site is among the earliest and most frequent alterations in cancer. Several studies suggest that aberrant methylation of the CpG sites of the tumor suppressor gene is closely associated with carcinogenesis. However, large-scale analysis of candidate genes has so far been hampered by the lack of high-throughput approach for analyzing DNA methylation. The aim of this study was to describe a microarray-based method for detecting changes of DNA methylation in cancer.