Open AccessReal-time determination of human telomerase reverse transcriptase mRNA in gastric cancer
Author(s) -
Lihua Hu
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i23.3514
Subject(s) - telomerase reverse transcriptase , telomerase , cancer , real time polymerase chain reaction , taqman , biology , reverse transcriptase , reverse transcription polymerase chain reaction , microbiology and biotechnology , messenger rna , cancer research , polymerase chain reaction , gene , genetics
To set up a real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay, to detect human telomerase reverse transcriptase (hTERT) messenger RNA in gastric carcinomas, and to evaluate quantitative determination of hTERT mRNA in the diagnostic value of gastric carcinomas, and to analyze the correlation between the expression level of hTERT mRNA and clinicopathological parameters in patients with gastric cancer.