Open AccessPolymerase synthesis and potential interference of a small-interfering RNA targeting hPim-2
Author(s) -
Shu-Qun Zhang,
Qingyou Du,
Ying Yang,
Zong-Zheng Ji,
Shengqi Wang
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i18.2657
Subject(s) - small interfering rna , microbiology and biotechnology , transfection , rna interference , gene silencing , t7 rna polymerase , messenger rna , biology , chemistry , rna , gene , biochemistry , escherichia coli , bacteriophage
To synthesize three small-interference RNAs (siRNAs) by T7 RNA polymerase-catalyzed reaction, and to investigate their efficacy on modulating the expression of serine/threonine kinase Pim-2 in human colon cancer cell line.