Open AccessBridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration
Author(s) -
Ailin Tao,
Shaoheng He
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i14.2103
Subject(s) - biology , microbiology and biotechnology , complementary dna , gene , primer (cosmetics) , genbank , recombinant dna , open reading frame , rapid amplification of cdna ends , gene expression , expression vector , genetics , molecular cloning , peptide sequence , chemistry , organic chemistry
To obtain the entire gene open reading frame (ORF) and to construct the expression vectors for recombinant allergen production.