Open AccessComparison of three PCR methods for detection ofHelicobacter pyloriDNA and detection ofcagAgene in gastric biopsy specimens
Author(s) -
Stella I. Smith,
Kolawole S. Oyedeji,
Arigbabu Ao,
Franck Cantet,
Françis Mégraud,
O. S. Ojo,
Uwaifo Ao,
JA Otegbayo,
S O Ola,
Coker Ao
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i13.1958
Subject(s) - caga , helicobacter pylori , polymerase chain reaction , rapid urease test , biopsy , gold standard (test) , biology , spirillaceae , gene , gastroenterology , virulence , microbiology and biotechnology , gastritis , pathology , medicine , genetics
To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and / or culture) in order to determine which of the three PCR methods (ureA, glmM and 26-kDa, SSA gene) was most appropriate in the diagnosis of Helicobacter pylori (H pylori ) infection and also to evaluate the detection of a putative virulence marker of H pylori, the cagA gene, by PCR in biopsy specimens.