Open AccessIsolation of murine hepatic lymphocytes using mechanical dissection for phenotypic and functional analysis of NK1.1+cells
Author(s) -
Chen Dong,
Haiming Wei,
Rui Sun,
Zhigang Tian,
Bin Gao
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i13.1928
Subject(s) - flow cytometry , microbiology and biotechnology , biology , intracellular , immune system , cell , digestion (alchemy) , concanavalin a , immunology , chemistry , biochemistry , in vitro , chromatography
To choose an appropriate methods for the isolation of hepatic lymphocytes between the mechanical dissection and the enzymatic digestion and investigate the effects of two methods on phenotype and function of hepatic lymphocytes.