Open AccessDifferential expression of cholangiocyte and ileal bile acid transporters following bile acid supplementation and depletion
Author(s) -
N. Sertac Kip
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i10.1440
Subject(s) - bile acid , symporter , medicine , cholangiocyte , g protein coupled bile acid receptor , biology , transporter , apical membrane , endocrinology , hepatocyte , taurocholic acid , fgf19 , cholestyramine , cyp8b1 , biochemistry , cholesterol , gene , receptor , membrane , fibroblast growth factor , in vitro
We have previously demonstrated that cholangiocytes, the epithelial cells lining intrahepatic bile ducts, encode two functional bile acid transporters via alternative splicing of a single gene to facilitate bile acid vectorial transport. Cholangiocytes possess ASBT, an apical sodium-dependent bile acid transporter to take up bile acids, and t-ASBT, a basolateral alternatively spliced and truncated form of ASBT to efflux bile acids. Though hepatocyte and ileal bile acid transporters are in part regulated by the flux of bile acids, the effect of alterations in bile acid flux on the expression of t-ASBT in terminal ileocytes remains unclear. Thus, we tested the hypothesis that expression of ASBT and t-ASBT in cholangiocytes and ileocytes was regulated by bile acid flux.