Open AccessA systematic study of the Thlaspi s.l. taxa in sections Nomisma, Thlaspi and Pterotropis from Turkey based on fruit morphological and molecular data
Author(s) -
Mehmet Cengiz Karaismailoğlu,
Behçet İnal,
Osman Erol
Publication year - 2022
Publication title -
acta botanica croatica
Language(s) - English
Resource type - Journals
eISSN - 1847-8476
pISSN - 0365-0588
DOI - 10.37427/botcro-2022-017
Subject(s) - taxon , biology , monophyly , clade , botany , phylogenetic tree , genus , population , zoology , biochemistry , demography , sociology , gene
The classification of Thlaspi s.l. is still problematic. Earlier phylogenetic research of the genus has focused on several small groups within Thlaspi s.str. and lacks detailed morphological observations. Therelationships among Eurasian taxa and the value of fruit morphology in defining them have yet to be studied. The aim of this study was to analyze 22 taxa belonging to the Nomisma, Thlaspi and Pterotropis sections of Thlaspi s.l. from Turkey using maximum likelihood (ML) analysis of Internal Transcript Spacer (ITS) sequences. We also analyzedtheir fruit morphological features. According to the results, the examined taxa fell into 2 main clades. Moreover, clade II showed 3 sub-clusters. Thlaspi huetii and T. aghricum were the most distant taxa with a distance of 0.49%; however, T. ochroleucum and T. violascens werefound to be 99% similar. According to ITS region data based on multiplepopulations of each taxon, T. arvense, T. huetii, T. perfoliatum, T. violascens, T. cataonicum, T. elegans, T. rosulare and T. aghricum were placed together in one cluster, which indicates that they are monophyletic. Thlaspi elegans was found to be a polyploid complex based on bootstrap (BS) (a resampling technique that uses replacement samplingto estimate statistics in a population) values, which varied widely among the studied T. elegans taxa (98, 65 and 49%). Fruit morphology also supported the inter-specific relationships based on molecular data,and relationships found by ITS region data were compatible with fruit type and geographic distribution. A diagnostic key based on fruit morphology is provided for the identification of the examined Thlaspi taxa.