Open AccessPhysiological responses of resistant and susceptible pepper plants to exogenous proline application under Phytophthora capsici stress
Author(s) -
Esra Koç
Publication year - 2022
Publication title -
acta botanica croatica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.284
H-Index - 22
eISSN - 1847-8476
pISSN - 0365-0588
DOI - 10.37427/botcro-2022-006
Subject(s) - phytophthora capsici , pepper , chlorophyll , proline , dpph , cultivar , malondialdehyde , carotenoid , horticulture , chlorophyll b , chemistry , chlorophyll a , biology , botany , food science , antioxidant , biochemistry , amino acid
Phytophthora capsici Leon. is the main pathogen that limits the production ofpeppers.In this study, the effects of 1 and 10 mM proline (Pro),prior to exposure of resistant (CM-334) and susceptible (SD-8) pepper seedlingsto P. capsici , on some physiological parameters were investigated. A lower Pro concentration (1 mM) was found to bemore effective than 10 mM Pro in increasing the stress tolerance of the CM-334cultivar. Namely, in CM-334 cultivar, the highest chlorophyll a , chlorophyll b ,carotenoid, glucose and fructose content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity percentage weredetected on the seventh day after application of 1 mM Pro + P. capsici , while the lowest malondialdehyde (MDA) amount was measured on the third day in the same treatment. The highest ferric reducing antioxidantpower (FRAP) increase was determined on the seventh day in the 10 mM Pro + P. capsici application. The effects of the same Pro treatments on the SD-8cultivar somewhat differed; the highest amounts of chlorophyll a , chlorophyll b , anthocyanins,fructose, totalprotein and endogenous Pro were detected on the seventh day in the 1 mM Pro + P. capsici application, while the lowestMDA amount was measured on the third day after the 10 mM Pro + P. capsici application, the highest DPPH% and FRAP values were detected on the seventh day with 10 mM Pro + P. capsici application. Although some differences were detected between thecultivars, Pro application against the P.capsici stress in general resulted in a positive effect on photosyntheticpigments, soluble carbohydrates and antioxidant capacity in pepper. Theexogenous application of Pro helped the non-resistant cultivar to overcome thestress.