Open AccessCrocus adamioides (Iridaceae) in the Bulgarian flora
Author(s) -
Tsvetanka Raycheva,
Kiril Stoyanov,
Samir Naimov,
Elena Apostolova
Publication year - 2020
Publication title -
acta botanica croatica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.284
H-Index - 22
eISSN - 1847-8476
pISSN - 0365-0588
DOI - 10.37427/botcro-2021-008
Subject(s) - iridaceae , phylogenetic tree , biology , taxon , crocus , flora (microbiology) , botany , internal transcribed spacer , population , zoology , evolutionary biology , geography , paleontology , genetics , demography , sociology , bacteria , gene
Crocus adamioides Kernd. et Pasche, as it is currently known, was originally treated as C. biflorus Mill. subsp. adamii B. Mathew in the flora of Bulgaria by Mathew (1982) and verified by Rukšāns (2017). The taxon was afterwards described as a separate species (Kerndorff et al. 2012), the holotype being collected in Kırklareli Province, European Turkey. The species was for the first time mapped in two floristic regions of Bulgaria. The diagnostic characters based on the general morphology and leaf anatomy were defined from the natural populations ofthe species and compared to the type specimen and relevant data from the literature. The phylogenetic position of the species was clarified by sequencing of the internal transcribed spacer region (ITS: ITS1 + 5.SsrDNA + ITS2) and comparison of the obtained sequence with those annotated in NCBI. A phylogenetic tree was built using Bayesian phylogeny. Results have shown the highest degree of phylogenetic similarity with C. adamioides from Turkey. The closest relative C. ranjeloviciorum Kernd., Pasche, Harpke et Raca remains in the proximity.Our morphological, anatomical and molecular analyses have revealed thatthe Bulgarian population shows a peculiar combination of characters specific to C. adamioides.