Open AccessPhenolic compounds in two subspecies of Drypis spinosa L. (Caryophyllaceae) in Croatia
Author(s) -
Dario Kremer,
Iztok Jože Košir,
Tanja Potočnik,
Nikolina Rogulj,
Karla Načinović,
Marko Randić,
Siniša Srečec,
Renata Jurišić Grubešić
Publication year - 2020
Publication title -
acta botanica croatica
Language(s) - English
Resource type - Journals
eISSN - 1847-8476
pISSN - 0365-0588
DOI - 10.37427/botcro-2020-015
Subject(s) - phytochemical , subspecies , capparis spinosa , botany , rutin , biology , flavonols , quercetin , traditional medicine , antioxidant , medicine , paleontology , biochemistry
As a contribution to chemotaxonomic relations, a quantitative analysis of bioactive phenolic compounds was carried out for the first time in Drypis spinosa L. subsp. spinosa and D. spinosa subsp. jacquiniana Murb.et Wettst. ex Murb. in Croatia. Total polyphenols (TP), tannins (T) andtotal flavonoids (TF) were determined in samples of leaves, stems, and roots using UV-Vis spectrophotometric methods. For the subsp. spinosa, the highest content of TF was in leaves (0.09%), as was the highest amount of TP (2.36%) and T (1.12%). In the subsp. jacquiniana, the highest contents of TF (0.10%), TP (1.96%), and T (0.88%) were measured in stems. Coumaric, ferulic and rosmarinic acid were identified and quantified by HPLC analysis in both subspecies. Quercetin and sinapic acid were identified only in subsp. spinosa, while rutin and naringenin were found only in subsp. jacquiniana. Among them, ferulic acid was identified only in flowers of both subspecies. The results of this studyrepresent a useful basis for further research of phytochemical and eventually phytotherapeutic potential of D. spinosa.