Open AccessPengaruh Variasi Waktu Fiksasi Sediaan Apus Darah Tepi (SADT) pada Pengecatan Giemsa terhadap Morfologi Sel Darah Merah
Author(s) -
Abdul Ghofur,
Tuti Suparyati,
Siti Fatimah
Publication year - 2022
Publication title -
jurnal kebidanan harapan ibu pekalongan
Language(s) - English
Resource type - Journals
eISSN - 2579-5481
pISSN - 2549-2772
DOI - 10.37402/jurbidhip.vol9.iss1.171
Subject(s) - fixation (population genetics) , giemsa stain , chemistry , medicine , pathology , biochemistry , gene
The Blood Smear is a slide whose one of the surfaces is coated using a thin layer of blood and stained with painting of giemsa or wright. Before painting, the preparation is fixated with methanol (methyl alcohol). Prolonged fixation should be avoided because methanol affects subsequent painting and causes some inhibition of intracellular enzymes. Methanol that is left too long in the open air will evaporate (decrease in concentration) and contain water so that it will affect erythrocyte morphology. This study aims to find out the effect of variations in sadt fixation time on giemsa painting on the morphology of red blood cells. This type of research is an experiment. The sample numbered 30 in the form of peripheral blood smear preparations divided by 3 based on variations in fixation time of 3 minutes, 5 minutes and 10 minutes. The results of the Chi-Square statistical test obtained a signification value of 0.00 3).