Open AccessAuto-hydrolysis of Tiquilia paronychioides (Phil.)A.T. Richardson and its relation to quercetin concentration
Author(s) -
Alejandrina M Llaure-Mora,
Mayar L. Ganoza-Yupanqui
Publication year - 2021
Publication title -
medicinal plant communications
Language(s) - English
Resource type - Journals
ISSN - 2452-4433
DOI - 10.37360/mpc.21.4.3.11
Subject(s) - quercetin , hydrolysis , chemistry , chromatography , high performance liquid chromatography , organic chemistry , antioxidant
The application of auto-hydrolysis of Tiquilia paronychioides (Phil.) A.T. Richardson aimed to determine the increase in quercetin concentration. Auto-hydrolysis was developed with aerial parts of T. paronychioides in aqueousmedium at 25°C with 20 rpm by 0, 2, 5, 8, 24, 48, 96 and 168 h, with 3 repetitions per test. Quercetin standards at concentrations of 5 to 80 ppm and auto-hydrolyzed extracts were evaluated by HPLC. The auto-hydrolyzed extracts showed Quercetin peaks from 8 h. Quercetin concentrations at 48, 96 and 168 h were 0.036 ± 0.0048, 0.033 ± 0.0105 and 0.026 ± 0.0068 mg quercetin/g, respectively, being statistically equal. In conclusion, the auto-hydrolysis of T. paronychioides generates the highest concentration of quercetin at 48 h.