Open AccessPhytochemical composition, antifungal activity, in vitro and in vivo toxicity of Syzygium cumini (L.) Skeels leaves extract
Author(s) -
Ernani Canuto Figueirêdo Júnior,
Yuri Wanderley Cavalcanti,
Andressa Brito Lira,
Hilzeth de Luna Freire Pessôa,
Wilton Silva Lopes,
Diego Romário da Silva,
Irlan Almeida Freires,
Pedro Luiz Rosalen,
Edja Maria Melo de Brito Costa,
Jozinete Vieira Pereira
Publication year - 2021
Publication title -
boletín latinoamericano y del caribe de plantas medicinales y aromáticas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 21
ISSN - 0717-7917
DOI - 10.37360/blacpma.21.20.5.40
Subject(s) - syzygium , phytochemical , toxicity , in vivo , chemistry , minimum inhibitory concentration , traditional medicine , biology , in vitro , biochemistry , botany , medicine , microbiology and biotechnology , organic chemistry
This study determined phytochemical composition, antifungal activity and toxicity in vitro and in vivo of Syzygium cumini leaves extract (Sc). Thus, was characterized by gas chromatography coupled to mass spectrometry and submitted to determination of Minimum Inhibitory (MIC) and Fungicidal concentrations (MFC) on reference and clinical strains of Candida spp. and by growth kinetics assays. Toxicity was verified using in vitro assays of hemolysis, osmotic fragility, oxidant and antioxidant activity in human erythrocytes and by in vivo acute systemic toxicity in Galleria mellonella larvae. Fourteen different compounds were identified in Sc, which showed antifungal activity (MIC between 31.25-125 μg/mL) with fungistatic effect on Candida. At antifungal concentrations, it demonstrated low cytotoxicity, antioxidant activity and neglible in vivo toxicity. Thus, Sc demonstrated a promising antifungal potential, with low toxicity, indicating that this extract can be a safe and effective alternative antifungal agent.