Open AccessPhenolic compounds of Tagetes lucida Cav. with antibacterial effect due to membrane damage
Author(s) -
Perla Y. Villa-Silva,
Anna Iliná,
Juan A. AscacioValdés,
Sandra Cecilia EsparzaGonzález,
Luis E. CobosPuc,
Raúl RodríguezHerrera,
Sonia Yesenia Silva Belmares
Publication year - 2020
Publication title -
boletín latinoamericano y del caribe de plantas medicinales y aromáticas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 21
ISSN - 0717-7917
DOI - 10.37360/blacpma.20.19.6.41
Subject(s) - antimicrobial , staphylococcus aureus , antibacterial activity , biology , comet assay , broth microdilution , traditional medicine , microbiology and biotechnology , pseudomonas aeruginosa , chemistry , bacteria , dna damage , minimum inhibitory concentration , biochemistry , dna , medicine , genetics
Tagetes lucida Cav. (Asteraceae=Compositae) is used for treating stomachinfections. The study focused on evaluating the composition and antimicrobial effect ofan extract of T. lucida Cav. The plant extracted with ethanol at 10% w/v, and theextract composition analyzed by Rp-HPLC-MS. The antibacterial effect was evaluatedagainst Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Salmonellacholeraesuis using disk diffusion, microdilution and bioautography methods. The sytoxand comet assays were used to evaluate the mechanism of action. In this way, ninephenolic compounds were detected in the extract of T. lucida. The extract exhibitedactivity only on S. aureus (MIC of 4.000 mg/ml). The bioautography revealed that thephenolic compounds could act synergistically. The sytox and comet tests showed anantibacterial action of the extract on the bacterial membrane and DNA of this bacterialstrain.