Open AccessEffect of encephalomyocarditis virus 3'Untranslated region on GFP transient expression: implications in recombinant protein production
Author(s) -
Marzieh Marzbany,
Mahsa Rasekhian
Publication year - 2020
Publication title -
boletín latinoamericano y del caribe de plantas medicinales y aromáticas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 21
ISSN - 0717-7917
DOI - 10.37360/blacpma.20.19.6.38
Subject(s) - untranslated region , messenger rna , three prime untranslated region , rna , biology , recombinant dna , protein biosynthesis , green fluorescent protein , five prime untranslated region , gene , virus , microbiology and biotechnology , gene expression , virology , genetics
The enrichment of therapeutic protein production yield in mammalian cell culturesby modulating mRNA stability is a fairly new strategy in biotechnological applications.Here, we describe the application of 3′-untranslated region (3′UTR) from RNA viralgenome to modulate mRNA stability. The data obtained showed that the use of the 3 'UTRsequence of the encephalomyocarditis virus (EMCV 3'UTR) downstream of the target genewas not able to significantly modulate the free energy density indicators of the RNA.However, the sequence influenced the stability of the mRNA (and, therefore, the amountof protein production) in a cell type and time-dependent manner, indicating a centralrole of mRNA-stabilizing binding sites/cellular factors in this process. Our data mightbe of interest for the biotechnology community to improve recombinant protein productionin mammalian cell cultures and RNA-based therapy/vaccination approaches.