Open AccessFifteen microsatellite markers for Herbertia zebrina (Iridaceae): An endangered species from South American grasslands
Author(s) -
Forgiarini Cristiane,
Curto Manuel,
StiehlAlves Eudes Maria,
Bräuchler Christian,
Kollmann Johannes,
Meimberg Harald,
Teixeira de SouzaChies Tatiana
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1700035
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , loss of heterozygosity , genetics , endangered species , population , genetic marker , evolutionary biology , allele , ecology , gene , demography , sociology , habitat
Premise of the study: Polymorphic microsatellite loci were developed and used to genotype individuals of Herbertia zebrina (Iridaceae) as a first step for assessment of intraspecific genetic diversity. Methods and Results: Primer pairs for 47 markers were developed: 20 from a microsatellite‐enriched library and 27 from a next‐generation sequencing run using the Illumina MiSeq platform. Of those, 15 loci were considered successful, of which 12 were polymorphic and three were monomorphic. The primers were tested in 50 individuals from three populations of H. zebrina . Two to 14 alleles per locus were identified, and observed and expected heterozygosity were 0.00–0.95 and 0.18–0.89, respectively. Tests of cross‐amplification to evaluate the applicability of these markers showed positive results in one congeneric species, H. darwinii , and in a phylogenetically closely related species, Calydorea crocoides . Conclusions: These microsatellite markers can be used for studies of genetic variation and genetic population structure, as well as to support conservation efforts.