Open AccessDevelopment and characterization of 18 polymorphic SSR markers for Barthea barthei (Melastomataceae)
Author(s) -
Huang Guilian,
Liu Haijun,
Sun Hongbin,
Liu Ying,
Zhou Renchao,
Liao Wenbo,
Fan Qiang
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600149
Subject(s) - biology , loss of heterozygosity , genetics , microsatellite , disjunct , genetic diversity , shrub , population , allele , expressed sequence tag , evolutionary biology , botany , gene , demography , genome , sociology
Premise of the study: To examine population differentiation, simple sequence repeat (SSR) markers were developed in Barthea barthei , a shrub with a disjunct distribution in the southern mainland of China and Taiwan. Methods and Results: We used Illumina HiSeq technology to sequence a genomic library for SSR identification. Twenty‐seven SSR loci were developed, of which 18 SSR loci were polymorphic in three populations composed of two varieties of B. barthei . At the population level, the number of alleles ranged from one to seven, and the observed and expected heterozygosity varied from 0 to 0.850 and from 0 to 0.809, respectively. Higher genetic differentiation between the two populations of B. barthei var. barthei ( F ST = 0.474) was observed relative to the two varieties ( F ST = 0.387 and 0.418, respectively). Conclusions: These polymorphic SSR markers may be useful for understanding phytogeographic history of B. barthei . Lower genetic differentiation between the two varieties than between the two populations of B. barthei var. barthei suggests that the taxonomic treatment may not hold.