Open AccessIdentification and characterization of microsatellite markers in Penstemon scariosus (Plantaginaceae)
Author(s) -
Anderson Chris D.,
Ricks Nathan J.,
Farley Kevin M.,
Maughan Peter J.,
Stevens Mikel R.
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500105
Subject(s) - biology , microsatellite , plantaginaceae , genetic diversity , genetics , evolutionary biology , taxon , allele , loss of heterozygosity , botany , gene , population , demography , sociology
Premise of the study: Penstemon scariosus var. albifluvis (Plantaginaceae) has been proposed to be federally listed as threatened due to its unique, geologically oil‐rich habitat. Developing simple sequence repeat (SSR) markers to study its genetic diversity would be most useful. Methods and Results: Using genomic reduction in combination with next‐generation sequencing, we identified SSR motifs with five to 15 perfect repeats in 1067 P. scariosus contigs. After multiple qualifying tests, 16 SSRs were selected for their robust polymorphic reliability across 12 taxa with as high as 21 alleles in a given taxon. With the exception of two monomorphic loci, the observed and expected heterozygosity values ranged from 0.083 to 1.000 and 0.398 to 0.920, respectively. Conclusions: These microsatellite markers will directly aid in studies of the genetic diversity and relatedness of P. scariosus, P. comarrhenus, P. compactus, P. cyananthus var. cyananthus, P. fremontii var. fremontii, P. fremontii var. glabrescens, P. gibbensii, P. strictus , and P. subglaber .