Open AccessMicrosatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)
Author(s) -
Weremijewicz Joanna,
Almonte Jasmin I.,
Hilaire Vanessa S.,
Lopez Frank D.,
Lu Stephen H.,
Marrero Sarah M.,
Martinez Catherine M.,
Zarate Edson A.,
Lam Ana K.,
Ferguson Samantha A. N.,
Petrakis Nicolas Z.,
Peeples Kelsey A.,
Taylor Ebony D.,
Leon Natalie M.,
Valdes Carolina,
Hass Michael,
Reeve Andrew B.,
Palow Danielle T.,
Downing Jason L.
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500095
Subject(s) - biology , orchidaceae , microsatellite , genetic diversity , loss of heterozygosity , population , threatened species , allele , ecology , zoology , demography , genetics , sociology , habitat , gene
Premise of the study: The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami's urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae). Methods and Results: Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity ( H o ) of 0.314 and average expected heterozygosity ( H e ) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average H o of 0.35 and an average H e of 0.224. For C. punctatum , allele numbers ranged from one to three, with an average H o of 0.257 and an average H e of 0.272. Conclusions: These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project.