Open AccessNuclear SSR markers for Miscanthus, Saccharum, and related grasses (Saccharinae, Poaceae)
Author(s) -
Hodkinson Trevor R.,
de Cesare Mariateresa,
Barth Susanne
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300042
Subject(s) - biology , miscanthus , germplasm , microsatellite , miscanthus sinensis , panicum , poaceae , locus (genetics) , botany , genetics , allele , microbiology and biotechnology , gene , bioenergy , biofuel
• Premise of the study: We developed nuclear simple sequence repeat (SSR) markers for the characterization of the biomass crop Miscanthus , especially M. sacchariflorus, M. sinensis , and M. ×giganteus , and tested for cross‐species amplification. • Methods and Results: Twenty‐nine SSR markers (di‐ and tetranucleotide repeats) were developed from DNA sequences obtained from 192 clones from an enriched genomic library of M. sinensis . All markers were successfully amplified in M. sacchariflorus, M. sinensis , and M. ×giganteus , and 19 amplified across a broad range of Miscanthus species. Polymorphism information content and expected heterozygosity values (19 locus sample) were 0.88 and 0.89, respectively, for M. sinensis , 0.48 and 0.54 for M. sacchariflorus , and were the lowest in M. ×giganteus (0.33, 0.41). Thirteen out of 19 primer pairs showed cross‐species amplification in non‐ Miscanthus sensu stricto taxa. • Conclusions: The new set of 29 SSR markers will be of high value for characterizing Miscanthus germplasm collections, for prebreeding, and for assessing variation in natural populations.