Open AccessMicrosatellite markers for Cariniana legalis (Lecythidaceae) and their transferability to C. estrellensis
Author(s) -
Tambarussi Evandro Vagner,
Sebbenn Alexandre Magno,
Moreno Maria Andréia,
Ferraz Elza Martins,
Kageyama Paulo Yoshio,
Vencovsky Roland
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200493
Subject(s) - biology , microsatellite , locus (genetics) , genetic diversity , genetics , allele , gene flow , genetic variation , gene , population , demography , sociology
• Premise of the study: Microsatellite primers were developed in the neotropical tree species Cariniana legalis (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow. • Methods and Results: We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT) 8 and (GT) 8 motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 C. legalis specimens and 26 C. estrellensis specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in C. legalis and three to 12 in C. estrellensis . For C. legalis , the observed ( H o ) and expected ( H e ) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For C. estrellensis, H o and H e ranged from 0 to 0.96 and from 0.14 to 0.91, respectively. • Conclusions: The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in C. legalis and the related species C. estrellensis .