Open AccessDevelopment and characterization of microsatellite markers for Alpinia oxyphylla (Zingiberaceae)
Author(s) -
Zou Ying,
Zou Pu,
Liu Huanfang,
Liao Jingping
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200457
Subject(s) - biology , microsatellite , zingiberaceae , loss of heterozygosity , locus (genetics) , genetic diversity , allele , population , alpinia , genetics , botany , evolutionary biology , rhizome , gene , demography , sociology
• Premise of the study: Microsatellite loci were isolated and characterized for population genetic studies of Alpinia oxyphylla (Zingiberaceae), a perennial rhizomatous herbaceous plant often used medicinally in China. • Methods and Results: A total of 85 loci were identified using a magnetic bead enrichment method, of which 23 were polymorphic. The level of polymorphism was characterized in 32 individuals from two populations; the number of alleles per locus ranged from 1 to 13; and observed heterozygosity and expected heterozygosity varied from 0 to 1 (mean: 0.6441) and 0 to 0.887 (mean: 0.6241), respectively. • Conclusions: The polymorphic microsatellite markers generated from this study will be useful for genetic diversity and structure analysis of A. oxyphylla .