Development and characterization of microsatellite markers for  Alpinia oxyphylla  (Zingiberaceae) | Zendy

Zou Ying | Zendy; Zou Pu | Zendy; Liu Huanfang | Zendy; Liao Jingping | Zendy

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Development and characterization of microsatellite markers for Alpinia oxyphylla (Zingiberaceae)

Author(s) -

Zou Ying,

Zou Pu,

Liu Huanfang,

Liao Jingping

Publication year - 2013

Publication title -

applications in plant sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.64

H-Index - 23

ISSN - 2168-0450

DOI - 10.3732/apps.1200457

Subject(s) - biology , microsatellite , zingiberaceae , loss of heterozygosity , locus (genetics) , genetic diversity , alpinia , allele , population , genetics , rhizome , evolutionary biology , botany , gene , demography , sociology

• Premise of the study: Microsatellite loci were isolated and characterized for population genetic studies of Alpinia oxyphylla (Zingiberaceae), a perennial rhizomatous herbaceous plant often used medicinally in China. • Methods and Results: A total of 85 loci were identified using a magnetic bead enrichment method, of which 23 were polymorphic. The level of polymorphism was characterized in 32 individuals from two populations; the number of alleles per locus ranged from 1 to 13; and observed heterozygosity and expected heterozygosity varied from 0 to 1 (mean: 0.6441) and 0 to 0.887 (mean: 0.6241), respectively. • Conclusions: The polymorphic microsatellite markers generated from this study will be useful for genetic diversity and structure analysis of A. oxyphylla .

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