Open AccessCharacterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)
Author(s) -
Werth Silke,
Cornejo Carolina,
Scheidegger Christoph
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200290
Subject(s) - biology , lichen , microsatellite , locus (genetics) , genetic diversity , population , allele , botany , genetics , gene , demography , sociology
• Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross‐amplification with L. immixta and L. macaronesica . The number of alleles per locus ranged from two to 23. Nei's unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria .