Characterization of microsatellite loci in the lichen fungus  Lobaria pulmonaria  (Lobariaceae) | Zendy

Werth Silke | Zendy; Cornejo Carolina | Zendy; Scheidegger Christoph | Zendy

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Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)

Author(s) -

Werth Silke,

Cornejo Carolina,

Scheidegger Christoph

Publication year - 2013

Publication title -

applications in plant sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.64

H-Index - 23

ISSN - 2168-0450

DOI - 10.3732/apps.1200290

Subject(s) - biology , lichen , microsatellite , locus (genetics) , genetic diversity , population , allele , botany , genetics , gene , demography , sociology

• Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross‐amplification with L. immixta and L. macaronesica . The number of alleles per locus ranged from two to 23. Nei's unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria .

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