Open AccessDevelopment of microsatellite markers in the hexaploid aquatic macrophyte, Myriophyllum spicatum (Haloragaceae)
Author(s) -
Wu ZhiGang,
Yu Dan,
Xu XinWei
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200230
Subject(s) - biology , myriophyllum , microsatellite , loss of heterozygosity , genetic diversity , locus (genetics) , allele , population , genetic structure , population genetics , botany , genetics , evolutionary biology , macrophyte , zoology , ecology , aquatic plant , genetic variation , gene , demography , sociology
• Premise of the study: We developed microsatellite primers to investigate genetic diversity and population genetic structure of the cosmopolitan submerged plant Myriophyllum spicatum . • Methods and Results: Twenty microsatellite loci were identified in M. spicatum using the microsatellite‐enriched library method. The numbers of alleles per locus ranged from one to 13, and the expected heterozygosity varied from 0 to 0.873 with a mean of 0.504 in two Chinese populations of M. spicatum . All of the loci were also found to be amplifiable in the related species M. verticillatum and M. sibiricum . • Conclusions: The results indicate that these markers will be significant for studies of population genetic structure and evolutionary history of M. spicatum as well as some of its related species.