Open AccessCost‐effective microsatellite markers for Banksia integrifolia (Proteaceae)
Author(s) -
Fatemi Mohammad,
Houliston Gary J.,
Haddadchi Azadeh,
Gross Caroline L.
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200130
Subject(s) - biology , proteaceae , microsatellite , evolutionary biology , population , genetic structure , botany , mating system , population genetics , genetic variation , genetics , allele , mating , demography , sociology , gene
• Premise of the study: Microsatellite markers were developed to assess the level of genetic variation and population structure in Banksia integrifolia , a widespread species endemic to eastern Australia. • Methods and Results: We used next‐generation sequencing approaches to identify and develop 11 polymorphic microsatellite markers with perfect tri‐ and tetranucleotide repeats. We tested these markers with 71 specimens from three populations. Observed and expected heterozygosities ranged from 0.0 to 0.875 and 0.0 to 0.763, respectively. • Conclusions: The developed markers will be valuable for studies of the population structure, mating system, and selection of provenances for restoration projects involving B. integrifolia .