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•  Premise of the study:  We report 11 primer sets for nine single‐copy nuclear genes in  Streptanthus  and other Thelypodieae (Brassicaceae) and their utility at tribal‐level and species‐level phylogenetics in this poorly resolved group.  •  Methods and Results:  We selected regions based on a cross‐referenced matrix of previous studies and public  Brassica  expressed sequence tags. To design primers, we used alignments of low‐depth‐coverage Illumina sequencing of genomic DNA for two species of  Brassica  mapped onto  Arabidopsis thaliana . We report several primer combinations for five regions that consistently amplified a single band and yielded high‐quality sequences for at least 70% of the species assayed, and for four additional regions whose utility might be clade specific.  •  Conclusions:  Our primers will be useful in improving resolution at shallow depths across the Thelypodieae, and likely in other Brassicaceae.

Single‐copy nuclear gene primers for Streptanthus and other Brassicaceae from genomic scans, published data, and ESTs