Open AccessSingle‐copy nuclear gene primers for Streptanthus and other Brassicaceae from genomic scans, published data, and ESTs
Author(s) -
Cacho N. Ivalú,
Strauss Sharon Y.
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200002
Subject(s) - brassicaceae , biology , clade , genetics , primer (cosmetics) , nuclear gene , gene , dna sequencing , expressed sequence tag , arabidopsis thaliana , computational biology , phylogenetics , evolutionary biology , genome , botany , chemistry , organic chemistry , mutant
• Premise of the study: We report 11 primer sets for nine single‐copy nuclear genes in Streptanthus and other Thelypodieae (Brassicaceae) and their utility at tribal‐level and species‐level phylogenetics in this poorly resolved group. • Methods and Results: We selected regions based on a cross‐referenced matrix of previous studies and public Brassica expressed sequence tags. To design primers, we used alignments of low‐depth‐coverage Illumina sequencing of genomic DNA for two species of Brassica mapped onto Arabidopsis thaliana . We report several primer combinations for five regions that consistently amplified a single band and yielded high‐quality sequences for at least 70% of the species assayed, and for four additional regions whose utility might be clade specific. • Conclusions: Our primers will be useful in improving resolution at shallow depths across the Thelypodieae, and likely in other Brassicaceae.